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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Hepatology. 2012 Dec;56(6):2375–2386. doi: 10.1002/hep.25900

Figure 1.

Figure 1

STAT5 regulates Nox4 expression through STAT5 binding to conserved GAS sites in the Nox4 gene promoter in liver. (A) Expression of Nox4, Puma, Bim and Socs2 was analyzed by quantitative real-time PCR in liver tissue from Stat5f/f and Stat5f/f;Alb-Cre mice. Values are shown as means ± SD. (B) mRNA expression of Nox4 and Socs2 in Stat5f/f and Stat5f/f;Alb-Cre mice. Mice were injected with GH, tissue was harvested after four hours and RNA was analyzed by quantitative real-time PCR. All values represent means ± SD. (C) Schematic of the Nox4 gene. Vertical boxes indicate exons and conserved GAS sequences are marked. Chromatin immunoprecipitation (ChIP) analysis of STAT5 binding to the putative GAS sites. Stat5f/f mice were injected with GH, tissue was harvested after 45 minutes and binding to GAS sites was analyzed by quantitative real-time PCR. DNA was amplified from STAT5-precipitated complexes using specific primers spanning GAS motifs in the Socs2 and Nox4 genes. All values represent means ± SD from 3 independent experiments performed in triplicates. (D) Levels of NOX4, PUMA and BIM in liver tissue from Stat5f/f and Stat5f/f;Alb-Cre mice. Expression of NOX4, PUMA and BIM was determined by western blotting. *P < .05; compared with corresponding controls.