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. 2012 Nov 15;26(22):2483–2498. doi: 10.1101/gad.201665.112

Figure 5.

Figure 5.

Mip120 is required to repress Gr63a misexpression. (A) Whole-mount antennae of wild-type and mip120 mutant (mip12067-21-6/mip12067-9A-9) expressing Gr63a-GAL4, UAS-mCD8∷chRFP. (B) Immunofluorescence using anti-Elav (pan-neuronal marker; green) and anti-RFP (red) of whole-mount maxillary palps of the genotypes indicated in A. (C) Whole-mount labellum of the indicated genotypes. (D) Number of Gr63a-expressing cells in A and B. n = 10 antennae or maxillary palps per genotype. (E) qRT–PCR using maxillary palp cDNA of indicated genotypes to measure Gr63a misexpression in the palp. n = 3 independent experiments per genotype. (F) Immunofluorescence using antibodies to nc82 (green) and RFP (red) on whole-mount brains of the genotypes indicated in A. (G) ChIP using antibodies to H3K4me3 or H3K9me2 on wild-type and mip120 mutant heads. n = 5–6 biological replicates per genotype. Data are presented as mean ± SEM. Two-tailed Student's t-test was used: (*) P < 0.05; (***) P < 0.001.