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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Nat Immunol. 2012 Oct 21;13(12):1213–1221. doi: 10.1038/ni.2454

Figure. 1. Jagged1 is a ligand for CD46.

Figure. 1

a and b, Analysis of the CD46 and Jagged1 interaction by ELISA. ELISA assays were performed utilizing either (a) immobilized soluble CD46 (CCP1-4) incubated with indicated recombinant soluble proteins (rhu J-1, recombinant human Jagged1; J-1DSL-EGF3, a recombinant protein containing the Notch1 binding site DSL and the first three EGF-like domains; N-111-13, a recombinant protein containing Notch1 EGF-like domains 11-13) or bovine serum albumin (BSA) or (b) immobilized J-1DSL-EGF3 incubated with indicated soluble proteins (sCR1, soluble recombinant complement receptor 1) in Ca2+ buffer (for further details on utilized proteins, see Supplementary Fig. 1). Mean ± SD (n = 5). (c) Analysis of Jagged1 binding to cell surface-expressed CD46. Red blood cells (RBCs) from CD46Tg mice express CD46 on the surface (left panel). RBCs from CD46Tg and from WT mice were incubated with biotinylated J-1DSL-EGF3 (middle panels) or BSA (right panel) and J-1DSL-EGF3-binding to RBCs measured using fluorochrome-labeled streptavidin. Shown is one representative data set of three independently performed experiments. *, p < 0.05; **, p < 0.005; ***, p < 0.001 when compared with BSA binding to immobilized protein.