Table 1.
ITC results for 7 and 8 binding to LTBh and El Tor CTB.
| Protein[a] | Ligand | +/− 2[b] | Kd [mm] | ΔH° [kcal mol−1] |
|---|---|---|---|---|
| LTBh | 7 | − | 7.5±1.9 | −5.8±0.5 |
| LTBh | 7 | + | 3.0±0.4 | −5.9±0.7 |
| El Tor CTB | 7 | − | 1.8±0.2 | −1.3±0.2 |
| LTBh | 8 | − | 5.0±0.5 | −8.5±0.9 |
| LTBh | 8 | + | 6.7±0.6 | −8.8±0.9 |
| El Tor CTB | 8 | − | binding not detected | binding not detected |
[a] Titrations were performed at 25 °C in phosphate-buffered saline using 100 μm LTBh/CTB subunit concentration. [b]+ indicates 200 μm GM1os 2 was added to the protein before titration with the ligand.