Figure 5.

Expression of VDR protein in AML cells exposed to 1,25(OH)₂D. AML cells after incubation for indicated times with 10 nM 1,25(OH)₂D were lysed and fractionated into the cytoplasmic (C) and nuclear (N) fractions. The lysates from equal numbers of cells were separated in SDS-PAGE and blotted to the membrane. The membrane was probed with anti-VDR. Actin was probed as a control of equal loading and transfer of proteins. (a) HL60 cells, (b) THP-1, MV4-11 cells, and (c) AML-M4 blasts from patient's peripheral blood. Based on [41, 42] and on unpublished data.