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. 2012 Sep 8;40(21):11126–11138. doi: 10.1093/nar/gks799

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Top trace shows the nucleoside composition analysis of GCGTACG**CATGCG obtained by enzymatic digestion with SVPD and alkaline phosphatase, followed by analysis by RP-HPLC at 260 nm. The bottom trace was recorded at 350 nm, where only 8-nitroguanine derivatives have a significant absorbance. The elution time for 8-nitroguanine is marked on the chromatogram. (G** = 8-nitro-2′-O-methylguanosine).