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. 2012 Sep 8;40(21):11126–11138. doi: 10.1093/nar/gks799

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Gel analysis of primer extension products synthesized by (a) AMV-RT and (b) human DNA polymerase β, from the control template containing 2′-O-methylguanosine. Varying concentrations of single deoxynucleoside triphosphates or an eqimolar mixture of all four dNTPs (dNTP mix) served as substrates for reactions as indicated. Lane M, 5′-[³²P]-labelled oligonucleotide size standards consisting of primer (P; 16-mer) and synthetic oligonucleotides generated by incremental addition of nucleotides, forming a 17-mer (+1), 18-mer (+2), 19-mer (+3) and 20-mer (+4). The +4 band corresponds to a primer that has been extended to the end of the template. Some 21-mer is visible in the dNTP mix reactions of AMV-RT, and is likely to result from non-templated terminal transferase activity of that enzyme (41). Band intensities are reported in the Supplementary Material.