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. 2012 Sep 8;40(21):11126–11138. doi: 10.1093/nar/gks799

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — MALDI-TOF mass spectrometric analysis of primer extension reactions. Reactions contained 1 mM each of dATP, dTTP, dCTP and dGTP and either a 2′-O-methylguanosine (OMe) or 8-nitro-2′-O-methylguanosine (8-N) template with AMV reverse transcriptase (AMV) or human DNA polymerase β (pol β). The mass spectra of these reactions are aligned with the spectrum of a mixture of oligonucleotides (20-mer std) representative of the fully-extended primers that would result from incorporation of one of the four dNTPs opposite 2′-OMeG or 8-nitro-2′-OMeG (20C, 20T etc.). 19-mer pol β products incorporating a C or an A opposite the modified nucleotide (19C, 19A) are identified by their calculated m/Z values. For clarity, the spectra have been expanded along the arbitrary y-axis by the factors indicated (2x, 3x etc.).