Table 2.

Melting temperatures (average of three determinations) of a family of DNA duplexes with and without the 8-nitroG modification

5′-GCGTACXCATGCG
3′-CGCATGYGTACGC
Y	X
	dG (°C)	2′-OMeG (°C)	8-NO2-2′-OMeG (°C)
C	63.6	61.0	52.4
T	55.3a	54.2	48.7
A	51.5a	50.0	49.9
G	54.9a	51.0	54.2

Conditions: 1 M NaCl, 0.1 M MgCl₂, 60 mM sodium cacodylate (pH 7), 3µM duplex. ^aTaken from reference (31).