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. 2012 Sep 8;40(21):10878–10892. doi: 10.1093/nar/gks844

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — The downstream DNA duplex is required for the activity of the mutants. (A) Shortened templates based on the −10−1/WT template. (B) Activity of the wild-type (wt) Eσ⁵⁴ decreases when the downstream DNA duplex is removed. (C) Shortened downstream DNA duplexes increase the intrinsic defects of the mutants.