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. 2012 Sep 8;40(21):10937–10949. doi: 10.1093/nar/gks832

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Validation of miRNA ‘hits’ in infected exosomes and cells. (A) Exosomes miRNA detection by TaqMan qPCR miRNA assay, miR-210 was used as a negative control for exosomal miRNA. Relative quantitation data represent mean ±SEM normalized to sno135 and sno202 using ΔΔC_t method. Fold change >1.5 or <1.5 is indicated with significance determined by Mann–Whitney U-test; *P < 0.05; **P < 0.01; ***P < 0.001 (n = 6–7 independent experiments, n.d. = not detected). (B) Cellular miRNA detection by TaqMan qPCR miRNA assay, miR-142-3 p was used as a negative control for cellular miRNA. Relative quantitation data represent mean ± SEM of normalized to sno135 and sno202 using ΔΔC_t method. Fold change >1.5 or <1.5 is indicated by the dashed line with significance determined by Mann–Whitney U-test; *P < 0.05; **P < 0.01 (n = 6–7 independent experiments, n.d. = not detected).