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. 2012 Sep 8;40(21):10950–10963. doi: 10.1093/nar/gks839

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — The expression of 4933440M02Rik is upregulated in the Alkbh1⁻^/⁻ and Tzfp^GTi/GTi testes. (A) Microarray analysis performed on whole testes indicating a 15-fold upregulation of the 4933440M02Rik gene in Alkbh1⁻^/⁻ testes. (B) Schematic representation of the 4933440M02Rik gene and transcript, a likely precursor for a piRNA cluster with id 1082 (adopted from http://pirnabank.ibab.ac.in). Magnification of indicated part of the cluster shows single piRNAs (light green) spliced from the piRNA precursor. The 10-bp Tzfp binding sequence (tbs) is located within ‘Exon 3’. (C,D) qPCR analyses of the 4933440M02Rik transcript in Alkbh1⁻^/⁻ (red) (C) and Tzfp^GTi/GTi (green) (D) indicate a dramatic upregulation in the mutant testes when compared to wild type (blue). (E) qPCR analyses of the 4933440M02Rik transcript in wild type (blue), Tzfp^GTi/GTi (green) and Alkbh1⁻^/⁻ (red) pachytene spermatocytes (PC) and round spermatids (RS). Wild type is used as reference sample (RQ = 1).