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. 2012 Sep 8;40(21):10950–10963. doi: 10.1093/nar/gks839

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Small RNA sequencing analysis reveals a majority of 29–30 nt long non-repeat intergenic piRNAs in pachytene spermatocytes. (A) Sequence length distribution of small RNAs in wild-type pachytene cells after high-throughput sequencing, indicating peak expression of 29–30 nt long small RNAs. (B) Distribution of small RNA classes in isolated pachytene cells from wild type (left), Alkbh1⁻^/⁻ (middle) and Tzfp^GTi/GTi (right). (C) Distribution of pachytene piRNAs into non-repeat associated, repeat associated (LTR, LINE and SINE) and other repeat sequences (analyses performed on the sample from (A)). (D) Distribution of non-repeat pachytene piRNAs into intergenic, exonic, intronic and UTR sequences (analyses performed on the sample from (A)).