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. 2012 Sep 8;40(21):10950–10963. doi: 10.1093/nar/gks839

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — A 4.2-kb segment of piRNA cluster 1082 makes up cluster 1082B and is regulated by Alkbh1 and Tzfp. Chromosomal view of cluster 1082 on chromosome 7 with back-to-back histograms illustrating piRNA reads in wild type (blue bars) compared to Alkbh1⁻^/⁻ (red bars) (A) and Tzfp^GTi/GTi (green bars) (B). After deep sequencing, cluster 1082 was redefined into the three separate clusters 1082A, 1082B and 1082C as indicated. Cluster 1082B is dramatically upregulated in mutant pachytene cells and corresponds to chromosomal position 132 472 367–132 476 593 containing 788 unique anti-sense piRNAs. A high-resolution view of a small segment of the upregulated region is given in (A) to illustrate individual, partially overlapping piRNAs.