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Sa Hfq binds RNA (A–N)3A sequences with high affinity. (A) Sa Hfq–(AG)3A-binding isotherm. (B) Sa Hfq–(AC)3A-binding isotherm. (C) Sa Hfq–(AU)3A-binding isotherm. (D) Sa Hfq–(AA)3A (or A7)-binding isotherm. The abscissa is the concentration of protein and the ordinate is millipolarization.
