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. 2012 Sep 8;40(21):11139–11154. doi: 10.1093/nar/gks806

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Repression by several chimeras shows dependence on the spacing between two operator-binding sites. β-galactosidase activity was measured in four strains of E. coli that express lacZ under regulation by the lacO^sym and lacO² operators. Because spacing between the two operators varies (X axis), chimeras that form dimer–dimer interactions are expected to have altered repression in the different strains. Repression was normalized to the ‘DEL’ no repressor condition; larger values correspond to tighter repression. Error bars show the standard deviation of the average. Dotted lines are to aid visual inspection of the data. (A) LacI-11, LLhG/E62K, and LLhT/V52A. (B) Wild-type LacI, LLhR, LLhA/Q55A, and LLhS/D62F. (C) LLhP, LLhF, and LLhE ‘3mut’ (I48V/Q55A/Q60R).