. 2012 Sep 8;40(21):11139–11154. doi: 10.1093/nar/gks806

Table 3.

Comparison of changes in in vivo repression compared to unmodified chimeras

	LacI	LLhG^a	LLhG/E62K^a	LLhP^b	LLhT	LLhA	LLhC	LLhE
Q55A		↓	↓	↓	↑	↑		↑ as 2mut or 3mut^c
Q55L		=	↓			↑
Q55I		↑					↑
V52A	↑^d		↑		↑		↑as 3mut^e

^aPreviously reported in (13). ^bPreviously reported in (12); LLhP and several of its variants showed bacterial toxicity, and thus the mutagenesis/selection protocol might have missed variants that enhance repression (e.g. at position 55). ^cThe Q55A mutation only enhanced repression in the presence of one or two additional substitutions (see ‘Results’ section). Q55A alone did not enhance repression. ^dPreviously reported in (17). ^eIn the presence of two additional substitutions (see the ‘Results’ section).