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. 2012 Aug 8;40(21):e166. doi: 10.1093/nar/gks738

Figure 3.

Figure 3.

Dox-dependent on/off regulation of EGFP expression. Embryos were the offspring of a cross of NmycEGFP and tTS transgenic mice. Pregnant mice were reared on drinking water containing 0 mg/ml dox (−dox) or 2 mg/ml dox (+dox). (a) Quantitative real-time PCR of EGFP expression in NmycEGFP and NmycEGFP:tTS E10.5d embryos. (b) Western blots of EGFP expression in NmycEGFP and NmycEGFP:tTS E10.5d embryos. (c) EGFP fluorescence of E10.5d embryos. The insets are bright field images. (d) Real-time PCR quantification of EGFP expression at 2 days postnatal (K: kidney; L: lung; H: heart; B: brain). (e) EGFP fluorescence in brain and kidney of postnatal 2 days mice (a feebly background fluorescence was observed in the kidney of wild-type mice.) The insets are bright field images. Scale bar is 1000 µm.