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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Immunol. 2012 Oct 24;189(11):5147–5154. doi: 10.4049/jimmunol.1200274

Figure 3.

Figure 3

TBI-induced MDSC express high levels of NO, ROS, and arginase. (A) Nitric oxide (NO) production (data represents the mean ± SD of triplicates * indicates p<0.05). OT-1 cells (1×105 cells per well) were stimulated in triplicates for 48h with OVA257-264 peptide in the presence of different ratios of MDSC and NO levels were measured, B) The levels of ROS production in MDSC from untreated or TBI-treated mice bearing B16 tumor was measured using DCFDA staining and flow cytometry. * indicates p<0.05. Data represents one of the three independent experiments C) Arginase activity was measured in untreated and TBI-treated mice bearing B16 tumors. Mean ± SD of two independent experiments is shown. * indicates p<0.01.