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. 2012 Apr 25;32(17):5973–5983. doi: 10.1523/JNEUROSCI.3873-11.2012

Figure 3.

Figure 3.

Phospho-ERK expression in C4 phrenic motor neurons after intrathecal EPO injection. A, Phospho-ERK (dark brown staining) is expressed in C4 phrenic motor neurons and is colocalized with CtB (top row, vehicle). pERK is upregulated after intrathecal EPO injection (middle row), and this upregulation is blocked by U0126 (UO; MEK/ERK inhibitor; second row from bottom) and the PI3K/Akt inhibitor LY294002 (LY; bottom row). B, Densitometry in CtB-labeled phrenic motor neurons showed increased phospho-ERK after intrathecal EPO- versus vehicle-treated rats. Pretreatment with U0126 and LY294002 brings pERK protein expression to vehicle levels. Data are means ± 1 SEM. *p < 0.05 versus all other groups. Scale bar, 100 μm.