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. 2012 Apr 25;32(17):5973–5983. doi: 10.1523/JNEUROSCI.3873-11.2012

Figure 4.

Figure 4.

Phospho-Akt expression in C4 phrenic motor neurons after intrathecal EPO injection. A, Phospho-Akt (dark brown staining) is expressed in C4 phrenic motor neurons and is colocalized with CtB back-labeled phrenic motor neurons. pAkt is upregulated after EPO injection (middle rows), and that upregulation is blocked after LY294002 or U0126 pretreatment (bottom two rows). B, Densitometry in CtB-positive phrenic motor neurons showed increased phospho-Akt after intrathecal EPO- versus vehicle-treated rats (p < 0.05). pAkt is at control levels after pretreatment with U0126 (UO) and after LY294002 (LY; p < 0.05). Data are means ± 1 SEM. *p < 0.05 versus EPO alone; #p < 0.05 versus vehicle. Scale bar, 100 μm.