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. Author manuscript; available in PMC: 2013 Mar 2.
Published in final edited form as: Cell. 2012 Mar 2;148(5):988–1000. doi: 10.1016/j.cell.2012.01.038

Figure 5. Hexadecenal Binds and Promotes BAX Activation.

Figure 5

(A) BAX (10 nM) was incubated with indicated lipids or detergents (0.1 and 1 mM) in the presence of BMH (100 nM) for 1 hr at 37°C before western blot.

(B) As in (A), but instead of oligomerization analysis, samples were extensively dialyzed, combined with bak-/-bax-/- HM, and incubated for 1 hr at 37°C before fractionation, and western blot for cyto c.

(C and D) Nitrocellulose membranes spotted with indicated picomoles of S1P or hex (combined with BSA and ponceau red for visualization) were incubated with either 50 ng of BCL-xL or BAX and probed with anti-BCL-xL or anti-BAX (horizontal mark for anti-BCL-xL, vertical for anti-BAX). The positive controls (*) on the blots are spots containing an IP antibody for BCL-xL or BAX.

(E) BAX (10 nM) was incubated with hexadecanal or hex (0, 0.1, 1, 5, and 10 μM) in the presence of BMH (100 nM) for 1 hr at 37°C.

See also Figure S5.