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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2012 Oct 4;32(12):2901–2909. doi: 10.1161/ATVBAHA.112.300208

Figure 1. Effect of Tobacco Smoke Exposure on Aneurysmal Dilatation in Animals Treated with Doxycycline.

Figure 1

Doxycycline (A) is effective in inhibiting AAA development in the smoke-free animals which underwent elastase perfusion. However, when the mice were exposed to tobacco smoke regularly beginning two weeks prior to aortic perfusion, doxycycline is no longer effective in preventing the aortic dilatation. Graphs (B and C) depict the relative aortic tissue expression of MMP-9 and MMP-12 mRNA in doxycycline-treated (n=4) and untreated (n=10) mice (B) and the relative mRNA expression in doxycycline-treated mice with (n=4) and without (n=6) tobacco smoke exposure (C). Zymography (D) was performed on protein extracts from 4 aortas taken from each experimental group. There was no effect of either doxycycline or smoke exposure on the zymographic bands produced by MMP-9 or MMP-2.