Figure 3.

Gfa2–VIVIT reduces microglial activation in Tg mice. A, Representative Western blots and mean ± SEM protein levels for the microglial marker Iba-1 from hippocampal homogenates of WT and Tg mice treated with vehicle and Gfa2–EGFP (Ct) or Gfa2–VIVIT (VIV). In the bar graph, Iba-1 levels are normalized to GAPDH internal controls and expressed as a percentage of the WT Ct group. Iba-1 levels were significantly reduced in VIVIT-treated Tg mice relative to Tg controls. Interestingly, the opposite effect was seen in WT animals (^#p < 0.01, *p < 0.05). Fluorescent labeling of Iba-1 in CA1 stratum radiatum of WT Ct (B), WT VIV (C), Tg Ct (D), and Tg VIV (E) mice corroborated these treatment effects. Scale bars, 250 μm.