Table 3.
Cytokine responses (mean ± SEM) from splenic CD4+ T cells stimulated in vitro with OVA (100μg/ml)a 7 days after the administration of either a s.c. or i.n. booster vaccination with 75 μg OVA + 1 μg LT
| Treatment Group | TNF-α | IFN-γ | IL-2 | IL-5 |
|---|---|---|---|---|
| ———(pg/ml/5 × 105 cells)——— | ||||
| s.c. vaccination | ||||
| AL (n=6) | 248.4 ± 32.4 | 8.4 ± 2.1 | 96.8 ± 27.1 | 187.5 ± 72.6 |
| AL+EX (n=6) | 355.6 ± 32.3b | 13.4 ± 1.7 | 139.3 ± 20.6 | 377.9 ± 89.4b |
| i.n. vaccination | ||||
| AL (n=10) | 811.2 ± 107.9 | 25.9 ± 4.9 | 149.5 ± 29.1 | 209.3 ± 79.1 |
| AL+ EX (n=10) | 1418.5 ± 115.7b | 36.1 ± 15.9 | 255.4 ± 57.4 | 453.2 ± 161.6 |
a
Con A stimulation (2 μg/ml) of isolated CD4+ T cells was used as positive control. In all cultures the amount of each cytokine produced exceeded 0.5 ng/ml/5 × 105 cells.
b
Significantly different from respective AL group (P<0.05)