Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Apr 17;5(6):1281–1294. doi: 10.1093/mp/sss036

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2012. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPB and IPPE, SIBS, CAS.

PMC Copyright notice

Figure 4. — Phenotypic Analyses of ΔPpDCL3/ΔPpDCL4 Double Mutants.

(A) Equal amounts of protonema tissue from wild-type (WT) and ΔPpDCL3/ΔPpDCL4 mutants were spotted onto solid medium and developed colonies were photographed after 3 weeks. ΔPpDCL3/ΔPpDCL4 mutants show accelerated gametophore development. Scale bar: 0.5 cm.

(B) WT and ΔPpDCL3/ΔPpDCL4 mutants display normal caulonema development after 10 d growth in darkness. Scale bar: 0.5 cm.

(C) Gametophore size is similar in WT and ΔPpDCL3/ΔPpDCL4 mutants. Scale bar: 2 mm.

(D) Sporophyte formation is rescued in ΔPpDCL3/ΔPpDCL4 mutants. Scale bar: 200 μm.

(E) RNA gel blots hybridized with antisense probes of miR390, miR156, and ta-siRNAs generated from the PpTAS3a precursor in WT and ΔPpDCL3/ΔPpDCL4 double mutants. An antisense probe for U6 snRNA served as control for normalization. Numbers under blots indicate normalized miRNA expression values with respect to WT.