Figure 5.

Neighboring miR156- and miR390-Sliced TAS Loci.

(A) An annotated genomic snapshot of PpTAS3a and PpTAS6a. Shaded regions indicate boundaries of indicated TAS loci, with locations and strand orientations of miRNA complementary sites and known functional ta-siRNAs shown. Insets above show the small RNA size distribution in the indicated genotypes, as well as the ‘phasing’ distributions of wild-type small RNAs in 21-nt bins (numbers on periphery indicate percentage of siRNAs in each bin). Browser track small RNA data (blue) and degradome data (magenta) show the 5' end positions from wild-type samples, with positive values indicating Watson-strand mapped reads, and negative values indicating Crick-strand mapped reads. Insets below show miRNAs aligned with miRNA complementary sites. Numbers above alignments are the number of degradome-derived 5' ends mapped to the 10th nucleotide of the alignment.

(B) Evidence for trans-acting slicing directed by the PpTAS6a-derived ta-siZNF. Annota

ted genomic snapshot showing protein-coding transcripts (gray), polyA+ RNAseq data (not strand-specific (Zemach et al., 2010), and degradome data. Inset below-left shows ta-siZNF/target alignment, with the number of degradome reads at the 10th nucleotide of the alignment indicated. Inset below-right shows schematic of the protein (from Phytozome).