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. Author manuscript; available in PMC: 2013 Nov 15.
Published in final edited form as: Free Radic Biol Med. 2012 Sep 12;53(10):1954–1959. doi: 10.1016/j.freeradbiomed.2012.08.597

Fig. 3.

Fig. 3

VPO1-mediated HOSCN generation. (A) pH-dependent HOSCN generation by VPO1. One hundred microliters of reaction containing 20 mM phosphate buffer, pH range from 4 to 8 as indicated, 500 nM rVPO1, 100 µM H2O2, 100 µM KSCN, and 100 µM TNB was carried out at 37 °C for 30 min. The absorbance at 412 nm was recorded. (B) VPO1 dose-dependent HOSCN generation. Experiments were carried out as in A at pH 5.5 and VPO1 concentration as indicated. (C) HOSCN dose-dependent generation by VPO1. Experiments were carried out as in A at pH 5.5. rVPO1 concentration was 500 nM and KSCN concentrations are indicated. The data are from three independent experiments. Error bars, SD.