Fig. 5.

Effects of substrate concentrations on VPO1 activity. Reactions were started by adding rVPO1 (100 nM, final concentration) to 20 mM phosphate buffer, pH 7.4, containing 10 µM H₂O₂, and varied concentrations of halides as indicated. Loss of H₂O₂ was continuously monitored with H₂O₂ electrode. The initial rates of H₂O₂ loss were measured over the first 10 s. The data are means and ranges of duplicate experiments.