Fig. 2.

Gating strategy; CD4⁺ and CD8⁺ T-cell subsets ex vivo. a Gating strategy used for all flow cytometry data shown; b–d frequency of activated CD4⁺ T-cell subsets in spleen and lymph nodes of wild-type (wt) and LLA-TG-3 mice; e–g frequency of activated CD8⁺ T-cell subsets in spleen and lymph nodes of wt and LLA-TG-3 mice; n = 14 for wt mice; n = 10 for no tumor and low tumor burden LLA-TG-3 mice; n = 13 for high tumor burden LLA-TG-3 mice. Depicted are mean values + SEM. *p < 0.05; **p < 0.005