Fig. 6.

Relative mRNA expression of TGF-β1 and IL-10. a The mRNA levels of TGF-β1 (left) and IL-10 (right) relative to wt splenocytes were analyzed using qRT-PCR and cDNA from splenocytes, lymph node cells, and tumor samples of wt and LLA-TG-3 mice; n = 1 for wt mice; n = 3 for low tumor burden LLA-TG-3 mice; n = 4 for high tumor burden LLA-TG-3 mice; b the mRNA levels of TGF-β1 and IL-10 relative to the immortalized melanocyte cell line B10.BR (calibrator) were analyzed using qRT-PCR and cDNA from the two LLA-TG-3 tumor cell lines Nu2 and 4046T. Depicted are mean values + SEM