Figure 2.

Immunohistochemical staining in the sepsis model. Liver tissue samples for immunohistochemistry were incubated to determine the levels of NF-κB after i.p. injection of LPS. A – LPS group; treated with LPS (2.5 ml) and then treated with ketamine (0.2 ml). B – Ketamine group; treated with 1 ml O₃/O₂ gas mixture for five days followed by LPS (2.5 ml) 24 h after the last infusion, and then an injection of ketamine (0.2 ml). C – O₃/O₂ + LPS + ketamine (DAB staining, 400×)