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. 2012 Nov 20;10(11):e1001428. doi: 10.1371/journal.pbio.1001428

Figure 6. Interspecific hybrids phenocopy piRNA pathway mutants in disrupted piRNA production and nuage mislocalization.

Figure 6

(A and B) Aberrant ping pong fractions in interspecific but not intraspecific hybrids. Ping pong fractions [23] for all TE families are compared between intraspecific hybrids and their parents (A) and interspecific hybrids and their parents (B). Only TE families that were represented by >50 small RNA reads and a ping pong fraction >0.1 in at least one of the three libraries are shown. (C) Ping pong fractions for TE classes derepressed in interspecific hybrids (taken from Figure 1A). (D and E) Increased antisense fraction of group III elements in interspecific hybrids. The antisense fraction of piRNAs derived from individual TE families in interspecific hybrids compared to D. melanogaster (D) and D. simulans (E). Classification of TE groups is from Li et al. [45]. Black line indicates equivalent antisense fractions in interspecific hybrids and parental pure species. (F) Anti-Aub and anti-Ago3 staining in D. melanogaster and interspecific hybrids in stage 2–6 egg-chambers. anti-Ago3 cross-reacts with D. simulans, but anti-Aub does not. Scale bars: 10 µm.