Figure 2.

Immunoblots of protein purified from cotton locules throughout development. Developmental stage in dpa is noted above each lane (DPA). The V-ATPase blot was probed with a mixture of antibodies raised to the 69-kD subunit and antibodies to the 57-kD subunit. Protein for the PM H⁺-ATPase blot was from microsomes (14 μg of protein per lane). Protein for the V-ATPase, PPase, and MIP blots was from tonoplast-enriched membranes (8 μg of protein per lane). Total soluble protein was used for blots probed with PEPCase and α-tubulin antibodies (50 μg of protein per lane).