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. Author manuscript; available in PMC: 2012 Nov 26.
Published in final edited form as: Free Radic Biol Med. 2010 Mar 6;48(12):1577–1587. doi: 10.1016/j.freeradbiomed.2010.02.040

Figure 1. A decrease in extracellular Zn causes a rapid and persistent increase in neuronal oxidant levels.

Figure 1

Cell oxidant levels were evaluated with the probe DHDCFDA after incubating differentiated PC 12 cells for 1-24 h in control media (black circles), or in chelated media containing 1.5 μM Zn (1.5 Zn) (white circles) or 15 μM Zn (15 Zn) (grey triangles). DCF fluorescence was normalized to the DNA content (propidium iodide, PI, fluorescence). Data was calculated considering the value of the control group at time 0 h as 1. Results are shown as the means ± SEM of at least four independent experiments. * Significantly different compared to C and 15 Zn at the same time point (p< 0.005).