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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Virol Methods. 2012 Jul 27;186(1-2):73–77. doi: 10.1016/j.jviromet.2012.07.024

Table 1.

EEHV qPCR standard curve assay parameters assessed using calibration curves.

qPCR assay Linear Dynamic Range (copies per reaction) Limit of Detection (100% Sensitivity) PCR % Efficiency R2 Slope Y-intercept
EEHV2 10 – 100,000 10 96.553 0.998 −3.407 38.508
EEHV3/4 10 – 100,000 10 100.483 0.999 −3.31 35.145
EEHV5 10 – 100,000 10 102.607 0.998 −3.261 37.037
EEHV6 10 – 100,000 10 94.186 0.998 −3.47 37.942