. Author manuscript; available in PMC: 2013 Dec 1.

Published in final edited form as: J Virol Methods. 2012 Jul 27;186(1-2):73–77. doi: 10.1016/j.jviromet.2012.07.024

Table 2.

Intra-assay accuracy of real-time PCR for EEHV1, 2, ¾, 5, and 6

Input copies	EEHV1		EEHV2		EEHV3/4		EEHV5		EEHV6

	Mean±SD^a	CV^b	Mean±SD^a	CV^b	Mean±SD^a	CV^b	Mean±SD^a	CV^b	Mean±SD^a	CV^b

10	35.51±0.43	1.23	34.66±0.66	1.92	32.02±0.60	1.87	31.38±0.37	1.18	34.40±0.62	1.79
10²	32.37±0.22	0.67	31.53±0.12	0.37	28.60±0.13	0.46	28.35±0.11	0.37	30.83±0.09	0.30
10³	28.86±0.06	0.21	28.25±0.18	0.62	25.27±0.10	0.40	24.89±0.07	0.29	27.55±0.09	0.33
10⁴	25.47±0.04	0.14	24.72±0.03	0.14	21.86±0.04	0.17	21.49±0.13	0.61	24.12±0.09	0.37
10⁵	22.10±0.04	0.18	21.35±0.04	0.20	18.62±0.07	0.37	18.09±0.14	0.76	20.87±0.04	0.18

Mean and standard deviation of the threshold cycle (Cq) from 5 replicates

Coefficient of variation