. Author manuscript; available in PMC: 2013 Dec 1.

Published in final edited form as: J Virol Methods. 2012 Jul 27;186(1-2):73–77. doi: 10.1016/j.jviromet.2012.07.024

Table 3.

Reproducibility of real-time PCR for EEHV1, 2, ¾, 5, and 6

Input copies	EEHV1		EEHV2		EEHV3/4		EEHV5		EEHV6
Input copies	Mean±SD^a	CV^b	Mean±SD^a	CV^b	Mean±SD^a	CV^b	Mean±SD^a	CV^b	Mean±SD^a	CV^b
10	34.43±0.14	0.40	34.73±0.81	2.34	31.77±0.26	0.81	30.90±0.19	0.60	34.71±0.36	1.02
10²	31.16±0.30	0.96	31.50±0.16	0.51	28.66±0.08	0.27	28.02±0.15	0.53	31.05±0.39	1.26
10³	27.67±0.26	0.93	28.34±0.45	1.58	25.39±0.15	0.61	24.67±0.07	0.27	27.71±0.34	1.21
10⁴	24.22±0.21	0.88	24.74±0.09	0.38	22.03±0.16	0.73	21.26±0.04	0.18	24.28±0.36	1.49
10⁵	21.52±0.19	0.87	21.39±0.09	0.40	18.68±0.14	0.74	17.82±0.03	0.19	20.85±0.16	0.80

Mean and standard deviation of the threshold cycle (Cq) from 5 independent experiments carried out between 1–3 months.

Coefficient of variation