Table 4.
Specificity validation of multiple real-time qPCR assays detecting known EEHV species.
| EEHV Speciesa | NAP No.b | Samplec | EEHV1 (Cq) | EEHV2 (Cq) | EEHV3/4 (Cq) | EEHV5 (Cq) | EEHV6 (Cq) |
|---|---|---|---|---|---|---|---|
| 1A | 31 | Blood | 23.7d | >40 | >40 | >40 | >40 |
| 1A | 33 | Blood | 28.7d | >40 | >40 | >40 | >40 |
| 1A/B | 19 | Blood | 27.9d | >40 | >40 | >40 | >40 |
| 1B | 45 | TWe | 16.4 | >40 | >40 | >40 | >40 |
| 2 | 12 | Blood | >40 | 29.5 | >40 | >40 | >40 |
| 3 | 27 | Colon | >40d | >40 | 26.4 | >40 | >40 |
| 4 | 22 | Blood | >40d | >40 | 26.6 | >40 | >40 |
| 5 | 50 | Blood | >40 | >40 | >40 | 33.3 | >40 |
| 6 | 35 | Blood | >40 | >40 | >40 | >40 | 36.5 |
| None | Nonef | Blood | >40 | >40 | >40 | >40 | >40 |
a
EEHV species previously detected in archival elephant DNA sample.
b
North American Proboscivirus (NAP) number assigned to the test sample. If no NAP number was previously assigned to the case, the elephant’s name is given.
c
Tissue or fluid from which the test DNA sample was obtained.
d
Previously published in Stanton et al. 2010.
e
Trunk wash
f
Negative control Asian elephant with no detectable EEHV in genomic DNA sample