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. Author manuscript; available in PMC: 2012 Nov 26.
Published in final edited form as: Free Radic Biol Med. 2011 Apr 30;51(2):480–489. doi: 10.1016/j.freeradbiomed.2011.04.028

Figure 3. Zinc deficiency induces an increase in tubulin oligomers in rat fetal brain, rat cortical neurons and IMR-32 cells.

Figure 3

Tubulin oligomers in 100,000 × g supernates were evaluated under the following experimental conditions. A-Tubulin oligomers in IMR-32 cells incubated for 24 h in IMR-32 cells in control non-chelated media (C) or in chelated media containing 1.5 μM Zn (ZD) without or with the addition of 0.5 mM LA (LA) or 1mM NAC (NAC) (left panel). Zn deficient supernates were treated 1.5 mM TCEP (center panel) for 30 min. B, C- 100,000 × g supernates isolated from (B) cortical neurons, and (C) fetal brain and liver, were incubated in the absence or the presence of 1.5 mM TCEP for 30 minutes. Tubulin oligomers in the supernates were assessed by separating proteins by SDS-PAGE under non-reducing conditions, and subsequent Western blot for β-tubulin. Tubulin oligomers of MW >100 kDA were quantitated. Results are shown as means ± SEM of 4 independent experiments for IMR-32 cells or 5-7 litters for GD19 brain. *Significantly different compared with the other groups (p<0.05, one-way ANOVA test).