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Immunoblots were probed using antibodies recognizing δ-zein. Lane 1 is a control with no cross-linking reagents added. In lanes 2 through 7, 1-g samples of starch granules were incubated with cross-linking reagents as follows: Caffeic acid levels, 0.02, 0.1, 0.2, 1, 2, and 10 mm, respectively; and potassium peroxymonosulfate levels, 0.005, 0.025, 0.05, 0.25, 0.5, and 2.5 mm, respectively. Each lane contained the total protein extracted from 5 mg of isolated granules.
