Table I.
Temperature | Protein
|
Reduction | |
---|---|---|---|
Control granules | Proteolyzed granules | ||
°C | % dry wt | % | |
50 | 0.33 ± 0.03a | 0.14 ± 0.02 | 58 ± 4 |
64 | 0.24 ± 0.03 | 0.13 ± 0.02 | 46 ± 5 |
Starch granules were incubated with thermolysin at a concentration of 0.4 μg mg−1 in 5 mm CaCl2. Hydrolysis was conducted at 64 or 50°C for 4 h. The granules were washed five times with water to remove residual thermolysin and then air dried. Control starches were treated in parallel at each temperature with thermolysin omitted.
Values are the average of three measurements.