Figure 1. Peptide glycosylation enhances the activation of antigen specific CD4+ T cell hybridoma.

A) O-glycan synthesis overview. B) IL-2 production from OVA specific CD4+ T cell hybridoma (BO.97.10) co-cultured with bone marrow derived DCs pulsed with two peptide variants with and without 2 and 4 glycan residues (GalNAc). Full length OVA used as a positive control. Individually cultured T cells and DCs had an OD value equal to the background.