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. 2012 Nov 26;7(11):e50413. doi: 10.1371/journal.pone.0050413

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© 2012 Assis et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Sequences were retrieved from GenBank and aligned using ClustalW method as implemented in the MEGA 4.0 program. The DMTV-2005 isolate showed a 10-nt deletion that leads to an early stop-codon and most likely results in non-functional protein production. The same deletion was observed in the Brazilian VACV Group 1 strains, including DMTV-2005. Nevertheless, the Brazilian Group 2 strains had no deletion in C23L. The deletion site is highlighted with a red box.