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. 2012 Jul 20;4(4):625–630. doi: 10.3892/ol.2012.815

Figure 4.

Figure 4.

Level of TGF-β in the supernatants 4 weeks after MLTC. The TGF-β levels in the supernatants 4 weeks after MLTC were analyzed by ELISA. In both the 1 and 5% Treg-added group, the TGF-β level in the supernatant was higher than that in the Treg-depleted group. The TGF-β level in the supernatant in the autologous tumor cell culture (L1023L) was 115 pg/ml. Given this background for the autologous tumor, it was determined that the high level of TGF-β in the supernatant in the Treg-added groups was derived from the autologous Tregs. Treg, regulatory T cell. MLTC, mixed lymphocyte-tumor cell culture; ELISA, enzyme-linked immunosorbent assay.