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. 2012 Nov 26;7(11):e46551. doi: 10.1371/journal.pone.0046551

Figure 2. Effects of miR-155 on inflammatory cytokine secretion.

Figure 2

PMA-induced THP-1 cells were transfected with miRNA mimic/inhibitor, and then incubated with oxLDL for 24 h. TNF-α and IL-6 expression levels were regulated after miR-155 mimic and inhibitor incubation. (A) The relative mRNA levels of TNF-α and IL-6. miR-155 was over-expressed via a single tail-vein injection of cholesterol-modified agomiR-155 into ApoE−/− mice. (B) ELISA detection of the protein levels of TNF-α and IL-6. (C) and (E) TaqMan RT-PCR was used to analyze the levels of IL-6 and TNF-α expression in vascular tissues, plasma, and BMMCsAnalysis using ELISA detected the protein levels of IL-6 and TNF-α in vascular tissues, plasma, and BMMCs in ApoE−/− mice (D) and (F) compared with the agomiR control. Mean ± SD calculated from five different experiments; *P<0.05 versus the sham or agomiR control group, **P<0.01 versus the “agomiR control” group.