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. 2012 Nov 26;7(11):e46551. doi: 10.1371/journal.pone.0046551

Figure 3. miR-155 suppresses atherosclerotic plaque progression, reduces macrophage accumulation, as well as decreases TNF-a and IL-6 levels in the thoracic aorta of ApoE−/− mice.

Figure 3

(A) Paraffin-embedded cross sections from agomiR control or agomiR-155-infused ApoE−/− mice were obtained throughout the thoracic aorta area and stained with hemolysin and eosin (HE). The sections were then imaged and quantified with Image-Pro Plus at a 400× magnification. (B) agomiR-155 reduces macrophage accumulation and MMP-9 expression, induces smoothe muscle cell composition (B,C, D) as well as decreases the TNF-a and IL-6 levels (C) within atherosclerotic plaques. Paraffin-embedded serial sections were stained with HE, rat anti-mouse CD68 antibody, and rat non-specific IgG (B) or rabbit anti-mouse TNF-a and IL-6 antibody, as well as rabbit non-specific IgG, respectively (C) and (D), at a 400× magnification. The aortic lesion size as well as CD68-, TNF-a-, and IL-6-positive lesion areas are shown from the agomiR control-infused group or agomiR-155-infused group. Data are represented as mean ± SD (n = 12). *P<0.05 compared with the agomiR control group.