Skip to main content
. 2012 Nov 26;7(11):e46551. doi: 10.1371/journal.pone.0046551

Figure 6. MAP3K10 is a functional target gene involved in the miR-155-mediated inflammatory effect on oxLDL-induced macrophages.

Figure 6

(A) MAP3K10 mRNA expression after transfection of small interference RNAs for MAP3K10 (siMAP3K10) or an insignificant control sequence (siNS) in oxLDL-induced macrophages as detected by RT-qPCR. (B) MAP3K10 protein expression detected by Western blot and quantification of Western blot. TNF-α and IL-6 protein levels were assessed by ELISA (C) and (D), as well as the phosphorylation and the total of the ERK1/2, p38, and JNK pathways measured by Western blot in siMAP3K10- or siNS-transfected cells (E) and (F). The miR-155 inhibitor-mediated inflammatory effect was rescued in oxLDL-induced macrophages via small interference RNAs for MAP3K10 (C), (D), (E), and (F). Data are presented as mean ± SD, n = 3; *P<0.05 compared with the siRNA control and # P<0.05 compared with the pure miR-155 inhibitor group.