Table 1. Distribution of Gb3 in organs from C57Bl/6 mouse obtained after staining with Alexa568-conjuguated 3E2.
Tissue | Gb3 | Isotypic control |
Abdominal fat | − | − |
Brain | − | − |
Colon | −* | −* |
Duodenum | − | − |
Esophagus | − | − |
Eyes | + | − |
Heart | − | − |
Kidney | + | − |
Liver | # | # |
Lung | − | − |
Muscles | − | − |
Ovaries | − | − |
Pancreas | − | − |
Pleura | − | − |
Skin | + | − |
Spleen | − | − |
Stomach | − | − |
Testis | + | − |
Thyroid | − | − |
Uterus | − | − |
Frozen healthy organs sections of 5 µm were hybridized with biotinylated 3E2 or its isotypic control, revealed by an Alexa 568-conjugated streptavidin and counterstained with DAPI. Pictures were observed under a confocal microscope (n = 3).
: High background.
: Lipofuscin autofluorescence.