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. 2012 Nov 26;7(11):e49780. doi: 10.1371/journal.pone.0049780

Table 1. Sequences of primers used for real-time PCR.

Target Primer sequence (5′–3′) Product (bp)
GAPDH Forward: TTCCACGGCACAGTCAAG 115
Reverse: ACTCAGCACCAGCATCAC
IFN-γ Forward: TCAACCCCTTCTCGCCACT 113
Reverse: GCTGCCTACTTGGTCCCTGA
TNF-α Forward: CGTCCATTCTTGCCCAAAC 94
Reverse: AGCCCTGAGCCCTTAATTC
IL-10 Forward: AGAACCACGACCCAGACATC 129
Reverse: CCACCGCCTTGCTCTTATTC
TGF-β1 Forward: AGGATCTGGGCTGGAAGTG 134
Reverse: CGGGTTGTGCTGGTTGTA
Leishmania Forward: TGT CGC TTG CAG ACC AGA TG 90
Reverse: GCA TCG CAG GTG TGA GCA C