Table 1. Sequences of primers used for real-time PCR.
Target | Primer sequence (5′–3′) | Product (bp) |
GAPDH | Forward: TTCCACGGCACAGTCAAG | 115 |
Reverse: ACTCAGCACCAGCATCAC | ||
IFN-γ | Forward: TCAACCCCTTCTCGCCACT | 113 |
Reverse: GCTGCCTACTTGGTCCCTGA | ||
TNF-α | Forward: CGTCCATTCTTGCCCAAAC | 94 |
Reverse: AGCCCTGAGCCCTTAATTC | ||
IL-10 | Forward: AGAACCACGACCCAGACATC | 129 |
Reverse: CCACCGCCTTGCTCTTATTC | ||
TGF-β1 | Forward: AGGATCTGGGCTGGAAGTG | 134 |
Reverse: CGGGTTGTGCTGGTTGTA | ||
Leishmania | Forward: TGT CGC TTG CAG ACC AGA TG | 90 |
Reverse: GCA TCG CAG GTG TGA GCA C |